I thought I would share an abstract photomicrograph I took last month. This is a microscopic view of Michigan maple syrup that was allowed to crystalize on a glass slide. The colors were due to cross-polarization. The horizontal field width of this image is 690 microns (0.69 mm., about 100X). 8 image stack.
All the technical mumbo-jumbo aside, I just thought it was pretty.
Click on the image below to see a larger view in my gallery.
For those of you who want to know the details, this was taken with a Nikon Eclipse Ci microscope, Plan 10X/0.25 NA lens, and a Nikon D300s digital camera. 1/20 sec, ISO 200. The image stack was aligned and stacked using the Zerene Stacker program. Post-processing was done with PhotoShop CS5.
#5. "RE: Micro Abstract - Michigan Maple Syrup" In response to Reply # 4
Ann Arbor, US
Thanks Mark, Yes, it is lit from below. I placed a linear polarizer on the light source that shines upward from the base of the microscope (the field lens). The second linear polarizer is on a slider above the objective turret. I rotate the first polarizer until the background gets dark and the colors just pop out of the sample. Colors vary based upon the chemical composition of the sample and its thickness. I did not use a coverslip on this prep so the final thickness is uneven - hence the different colors. This type of abstract is an acquired taste. The maple syrup however, is always good.