Whether you shoot RAW or Best size/quality JPEG, you can set your in-camera sharpening up to 6 or 7 (factory has it set around 3). You can also set the Active-DLighting to High, and gain 2 stops of detail in highlights, without any loss of image quality.
Turn OFF High ISO noise reduction and Turn off long exposure NR as well. I have found them unnecessary, but your experience could be different...
Take your time in shooting to see how the camera behaves. I used a D80/D200 before the D7000.
The D7000 is incredible. The shutter is very quiet (in normal mode!) the sensor is very revealing. I found the transition from the D200 to the D7000 pretty seamless. You will find the D7000 feels more solid than the D80. There are a ton of features (many of which I haven't used because I haven't opened the manual yet).
The focus system is more advanced than what you're coming from, but the options on the D7000 seem more similar to the D80 than the D200. Also, the high ISO capability is miles ahead of the D80.
In the end, it's a camera. Keep that in perspective, and in all likelihood you'll make some great images.